RECOMBINANT ORGANISMS AND METHODS FOR PRODUCING GLYCOMOLECULES WITH LOW SULFATION

摘要

The invention provides a recombinant Labyrinthulomycetes cell for the production of a low sulfate glycomolecule. The cell comprises a nucleic acid encoding a heterologous glycomolecule, and a sequence encoding a heterologous oligosaccharyltransferase. The cell produces the heterologous glycomolecule having fewer sulfated glycans compared to the same heterologous glycomolecule produced by a corresponding cell not comprising the heterologous oligosaccharyltransferase. The cells advantageously produce and, optionally secrete, the heterologous glycomolecule. Thus, the invention provides recombinant organisms that provide glycomolecules having a glycosylation profile that is more similar to the glycosylation profile produced in mammalian cell.