PHOTOTOXICITY TESTING METHOD BASED ON RECONSTRUCTED SKIN MODEL

摘要

Disclosed is a phototoxicity testing method based on a reconstructed skin model, comprising the following steps. S1: receiving and restoring a reconstructed skin model; S2: selecting a light source; S3: determining a maximum tolerated dose of the reconstructed skin model; and S4: testing the phototoxicity of a test sample. The phototoxicity testing method based on the reconstructed skin model can be performed for water-soluble chemicals and cosmetic raw materials, as well as oily and creamy cosmetic products and formulated substances in different proportions. The present method not only simulates changes due to biological reactions of human skin in response to different types of exogenous substances, but also broadens the testing range of the substances to be tested, in that the substances to be tested are no longer limited to water-soluble chemical substances and may comprise finished cosmetics and formulations with various properties and forms.