The present invention comprises the steps of: (i) irradiating an electron beam to a chitin-based solid material; (ii) washing the chitin-based material subjected to electron beam irradiation; (iii) adding a basic solution to the washed chitin-based solid material; And (iv) preparing a suspension containing chitin nanocrystals by homogenizing a chitin-based solid material dispersed in an aqueous system to which a basic solution is added to prepare a suspension containing chitin nanocrystals.
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