Disclosed is a method of separating the mucosa and submucosa from the proximal muscle at a site of an organ of a subject, wherein the organ is not the esophagus. In some embodiments, the organ is in the gastrointestinal tract. Such a method includes submucosal injection of a pharmaceutical composition comprising an extracellular matrix (ECM) hydrogel into an organ of a subject to form a cushion between the submucosal and underlying proximal muscles at the site of the organ, wherein ECM hydrogel The gel has the following characteristics: a) 50% gelation time of less than 30 minutes at a temperature of about 37°C; b) a flow viscosity suitable for injection into the trachea; And c) a hardness of about 10 to about 400 Pascal (Pa).
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