A method for obtaining a quantum efficiency distribution, a method for displaying a quantum efficiency distribution, a program for obtaining a quantum efficiency distribution, a program for displaying a quantum efficiency distribution, a fluorescence spectrophotometer and a display device

摘要

There is provided a method of obtaining a quantum efficiency distribution, comprising: irradiating a reference material with excitation light having a first range of wavelengths; Obtaining, by taking an image of the reference material with a photographing device which comprises at least one first channel corresponding to the first wavelength range and a second channel corresponding to a second wavelength range, an irradiation luminance value of the first channel and an irradiation luminance value of the second channel in each pixel of the image of the reference material; Irradiating the predetermined surface of the sample with the excitation light; Obtaining a measurement luminance value of the first channel and a measurement luminance value of the second channel in each pixel of an image of the predetermined surface by capturing the image of the predetermined surface with the photographing device; Calculating an absorption luminance value from a difference between the irradiation luminance value of the first channel and the measurement luminance value of the first channel; Calculating a fluorescence luminance value from a difference between the irradiation luminance value of the second channel and the measurement luminance value of the second channel; Calculating a quantum efficiency of each pixel based on the irradiation luminance values, the absorption luminance value and the fluorescence luminance value; and obtaining the quantum efficiency distribution on the predetermined surface of the sample.