A procedure of extraction and purification of a pancreatic hormone acting on the respiratory centers (Machine-translation by Google Translate, not legally binding)

摘要

Improvements introduced in the object of the main patent no. 222,411 by means of a procedure for the extraction and purification of a pancreatic hormone that acts on the respiratory centers, characterized in that the ox pancreas is subjected to the treatment of an acidic alcoholic mixture, and then obtained by global precipitation to the salt of insulin, vagotonin, and the hormone active on the respiratory centers, as well as various impurities, proceeding then to the separation of the insulin by precipitation of the latter to its iso-electric point, then recovering by precipitation to the lithium chloride in an acid medium the vagotonin and the said active hormone on the respiratory centers, proceeding then to the total elimination of the vagotonin by dialysis and to a fractionation to the alcohol of the obtained pancreatic extract, being finally subjected the extract to this fractionation to the lithium chloride, drying it to the alcohol ether and under vacuum. (Machine-translation by Google Translate, not legally binding)