Method of and apparatus for collecting fractions of liquids

摘要

1,064,575. Collecting chromatographic fractions. HEINRICH MACK NACHF. June 24, 1965 [June 25, 1964], No. 26893/65. Heading B1X. [Also in Division F4] Liquid fraction from a chromatography column are collected by forming the fractions into drops and allowing the drops to fall successively into a refrigerated tube so that the first drop spreads out and freezes on the bottom of the tube and subsequent drops from frozen layers one above the other. As shown, the tube into which the drops fall is surrounded by a cooling medium 7 in a vacuum flask 6 having an insulated lid 5, the tube having a push-on bottom 4. The means for supplying the drops to the tube (Fig. 3, not shown) comprises a cylindrical collector (8) having an upper inlet opening (9) for receiving liquid from the chromatography column and a siphon (10) connected to its lower end. In the case of natural or artificial dyes or coloured proteins the fractions can be recognised in the frozen column by their colour and separated by sawing. Alternatively the column may be placed on photo-sensitive paper and exposed to ultra-violet light, the fractions being distinguishable on the developed paper because of their different light absorbing properties and being again separated by sawing. The solid constituents of the column may be obtained by freeze-drying (see Division F4).