method of manufacture of so-called anti inflammation and certain virussairauksiin favourably the active drug used for the happamissa circumstances saostumatonta kserosinfraktiota kserosinin käymisliem

摘要

1,186,598. Antibiotic xerosin. CARTERWALLACE Inc. 16 June, 1967 [27 July, 1966], No. 27771/67. Heading C2A. An active fraction of the anti-inflammatory, antiviral antibiotic xerosin is obtained by (a) separating the whole cell material from a fermentation broth of Achromobacter xerosis I.M.U.R. 134, (b) autolyzing or mechanically rupturing the solid cell material to obtain a mixture of spent cell material in a liquid phase, (c) separating spent cell material from the liquid phase, (d) acidifying to pH 2 to. 4, (e) collecting the acid-insoluble precipitate, (f) neutralizing the liquid, (g) adding a precipitating agent which is a water-miscible organic solvent (A) in which the fraction is insoluble, e.g. acetone, dioxane, ethanol or isopropanol or is a neutral water-soluble inert inorganic salt (B) e.g. (NH 4 ) 2 SO 4 , and separating the precipitate. Alternatively, in step (g) the xerosin fraction is precipitated by precipitant (A), dissolved in water and re-precipitated by precipitant (B). The process is applicable to (a) separated cells, (b) clarified broth, and (c) whole broth containing separated cells from a previous batch of whole broth. The xerosin fraction is an amorphous solid slightly soluble to very soluble in water (depending upon purity), very soluble in acid solutions and substantially insoluble in organic solvents such as benzene, chloroform, ethanol, acetone dioxane, isopropanol and pyridine.