Reagent formulation and the test strip for adjusting an acidic ph - region in the detection of substances in aqueous liquids (9) to

摘要

1,182,604. Detecting urobilinogen, mesobilirubinogen and bilirubin. MILES LABORATORIES Inc. 9 March, 1967 [11 March, 1966], No. 10861/66. Heading B1X. A two component test pack for detecting urobilinogen and mesobilirubinogen in an aqueous liquid .comprises as the first component para-di(lower alkyl)aminobenzaldehyde and a water-soluble solid inert acid, .and as the second component a solid substance which decomposes on contact with the mixture obtained by adding the first component to the aqueous liquid to produce an inert solvent for any coloured substance formed by reaction of the para-di(lower alkyl)aminobenzaldehyde with urobilinogen or mesobilirubinogen, the inert solvent being substantially immiscible with the aqueous liquid and having a density different to that of the mixture. The "lower alkyl" group may be C 1 to C 4 ; para-dimethylaminobenzaldehyde is preferred. The acid may be sulphosalicylic, maleic, oxalic, mesoxalic, acetylene dicarboxylic, monochloromalonic or monobromo malonic acid. The second component may be magnesium . or aluminium alkoxides, preferably of C 4 to C 8 alkanols, for example aluminium sec-butoxide, n-hexyloxide, n-heptyloxide, magnesium n-pentyloxide, n-hexyloxide, n-heptyloxide; or alkali metal or ammonium salts of C 5- C 10 aliphatic acids, for example sodium nonanoate, iso decanoate, octanoate and isoctanoate. With maleic acid or sulphosalicylic acid may be used sodium bicarbonate to assist dissolution. Bilirubin may be determined by first reducing it to mesobilirubinogen. Reference has been directed by the Comptroller to Specification 779,921.