REACTION SYSTEM FOR SEQUENTIALLY TRANSFERRING REAGENTS FROM A PLURALITY OF STORAGE VESSELS

摘要

1,235,726. Dispensing measured quantities of liquids. DANISH INSTITUTE OF PROTEIN CHEMISTRY. 17 Sept., 1968 [18 Sept., 1967], No. 44160/68. Heading B8N. [Also in Divisions B1-B2] A reaction system for use for example in the solid state synthesis of peptides comprises a plurality of first vessels 111a-111c for reagents, a second vessel 112 which may be a metering vessel, a plurality of conduits 115a- 115c free of valves and each connecting one first vessel to the second vessel, and an automatic control system for controlling the flow of reagents from the first vessels to the second vessel by sequentially selectively applying to the reagents in the first vessels 111a &c. by means of gas pressure in lines 122, 116a &c., a first pressure which is higher than the pressure in the second vessel 112 so that reagent flows into the second vessel, and a second pressure which is lower than the pressure in the second vessel so that reagent ceases to flow to the second vessel and flows back from the conduits to the first vessels, by siphon action when the second pressure is atmospheric pressure or by applying vacuum as the second pressure. In the embodiment shown, reagent passes to metering vessel 112 until the liquid level in side-arm 131 reaches photo-cell 134 or 135, when reagent flow ceases. Solenoid valve 133 is operated to allow the reagent to flow to reaction vessel 113, completion of flow is signalled by photocell 140. Shaker 144 is operated to mix the reactants. Solenoid valve 147 is then opened to allow the liquid to be filtered through disc 145 into waste flask 114. The second reagent is then passed into the reaction vessel. The reaction system is under the control of unit 154 with tape reader 155.