A method for obtaining a sterile and active zymosan from the outer shell of the yeast cells, characterized in that: a) the yeast is selected microscopically from living cells; b) treat said yeast with an aqueous sterile, hot, 0.5 molar disodium phosphate solution, stirring until a uniform suspension is formed and then filtering to obtain a clean and loose suspension, continuing the hot stirring at about 100º c. During 3 hours; c) cooling to about 37ºC and adjusting the ph between 7.8 and 8; d) add an antibiotic or a mixture of several antibiotics, trypsin and toluene, gradually while digested in an oven, at a controlled temperature for 17 days, keeping the ph constant; e) centrifuge the product at appropriate speed and separate the supernatant liquid by washing with sterile boiling distilled water, stirring vigorously and centrifuging each time, repeating the operation as many times as necessary until the absence of carbohydrates and proteins in the washing liquid; f) the sediment is mixed with 99% ethanol, stirring, centrifuged and the washing is repeated now with a half volume of ethanol, and dried under vacuum; g) the dry powder is mixed with 99% ethanol, stirred for several hours and centrifuged and dried in vacuum; h) a mixture is prepared with 100 c.c,, of 99% ethanol and 2 c.c. Of hydrochloric acid of density 1,8 and 22ºc bº, it is cooled to -70ºc and at this temperature it is mixed with the powder obtained in the front cover, stirring and stirring is maintained for several hours at the same temperature; i) is centrifuged and dried under vacuum by repeating steps h) and i) 3 times, always at -70º c; j) 99% ethanol alkalized with ammonia is added to the residue, stirring and maintaining the suspension for several hours, at -70º c, then centrifuging; k) 99% ethanol is added to the sediment, centrifuged again and dried under vacuum. (Machine-translation by Google Translate, not legally binding)
展开▼
