Ovular and seminal cell extracts - having a haploid chromosome set for therapeutic and diagnostic use

摘要

Prepn. and use in therapy and diagnostic use and ovular cell extracts having a haploid chromosome set from corpuscular and high molecular fractions (nucleic) acids, proteins, lipides and polysaccharides) and their degradation products, for aiding cell-proliferation and treating sterility and fertility. Antibody-sera against fractions with X- or Y-chromosomes can be used for sex-determination. Cells from fish are esp. suitable as starting materials. In example, for an antibody-serum against bovine Y-chromosomes cell-nuclei are obtained by grinding oval cells from trout-roe in liquid N2, centrifuging in a saccharose-gradient, freeze-drying and decomposing chemically. Antibody serum from rabbits immunised with the oval product is incubated with bull-sperm. The non-agglutinated sperm-cells are isolated and the nuclei from the spermatozoa with Y-chromosomes used for the active immunisation of rabbits or sheep.