METHOD FOR PREPARING INHIBITOR-FREE, EASILY WATER-SOLUBLE ENZYMES AND THEIR USE IN DETERGENT COMPOSITIONS

摘要

A method for obtaining inhibitor-free and easily water-soluble enzymes, which involves grinding animal pancreas that has been frozen immediately after slaughtering the cattle, extracting said material with an alcohol containing 1-2 carbon atoms, treating said extract with a a water-insoluble liquid solvent selected from the group consisting of a butyl alcohol and an ether, causing said enzyme to precipitate from the resulting clear layer with a water-soluble solvent selected from the group consisting of an alcohol containing 1-2 carbon atoms and a ketone at a temperature below 10ºC, and recover the enzyme. (Machine-translation by Google Translate, not legally binding)