THYROXINE DETERMINATION METHOD EMPLOYING THE COMPETITIVE PROTEIN BINDING TECHNIQUE

摘要

A method for use in determination of thyroxine in biological fluids which comprises extracting thyroxine with tetrahydrofuran and separating thyroxine from the precipitated proteins and then from the tetrahydrofuran, and thereafter equilibrating with thyroxine binding globulin and radioactive labeled thyroxine, removing unbound thyroxine and determining the radioactive labeled thyroxine by counting techniques.