method for carrying out enzymatic reactions using aqueous two-phase systems (fertilizer

摘要

1461841 Enzymic reactions in biphasic aqueous-organic systems SNAMPROGETTI SpA and ISF SpA 4 Jan 1974 [26 Jan 1973] 00502/74 Headings C2U and C2C [Also in Division G1] Enzymic reactions using one or more of laccase, hydroxy-steroid dehydrogenase, lipase, lactate dehydrogenase and alcohol dehydrogenase, in which the substrate is insoluble or only sparingly soluble in water are performed in a 2-phase system comprising water and an organic solvent immiscible with water and miscible with the substrate. Suitable solvents are esters of C 2-15 carboxylic acids, alkyl ethers, C 4-10 alcohols, aliphatic, cycloaliphatic and aromatic hydrocarbons and halogenated hydrocarbons. Substrates specified are estradiol, estrone, estriol, equilin, equilenin, diethyl stilbestrol, testosterone (giving 4-androstene-3,17- dione), olive oil (giving fatty acids) and butanol (giving butyric aldehyde).