METHOD OF IMMUNIZATION FOR MYCOPLASMA RESPIRATORY INFECTIONS OF LIVESTOCKS,A SUSPENSION VACCINE THEREFOR AND A PROCESS FOR PREPARING SAID SUSPENSION

• 1. 一种提高人体免疫力的鲜活小球藻悬浮液的制备方法 [P] . 中国专利： CN108504580A . 2018-09-07
• 2. 包含表达偏肺病毒(hMPV)抗原的重组活BCG的免疫原性制剂，其以无需佐剂地从冻干物制备的悬浮液形式存在并且适合于其制药用途 [P] . 中国专利： CN105745323B . 2020.09.04
• 3. METHOD OF IMMUNIZATION AGAINST MYCOPLASMA RESPIRATORY INFECTIONS OF LIVESTOCK A SUSPENSION VACCINE THEREFOR AND A PROCESS FOR PREPARING SAID SUSPENSION [P] . IL43310A . 1978-01-31

  机译： 悬吊疫苗的家禽肺炎的免疫接种方法及其制备方法

• 4. METHOD OF IMMUNIZATION AGAINST MYOCOPLSMA RESPIRATORY INFECTIONS OF LIVESTOCK, A SUSPENSION VACCINE THEREFOR AND A PROCESS FOR PREPARING [P] . IL43310B . 1978-01-31

  机译： 禽流感的预防方法，一种悬吊疫苗及其制备方法

• 5. Lyophilized composition, compound and aqueous vaccine composition composed of lyophilized composition, vaccine composition, method of immunizing a vertebrate by vaccination, method for preparing a lyophilized composition and an aqueous colloidal suspension [P] . BR9912067A . 2001-04-10

  机译： 冻干组合物，由冻干组合物组成的化合物和含水疫苗组合物，疫苗组合物，通过疫苗免疫脊椎动物的方法，冻干组合物的制备方法和水胶体悬浮液

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• 1. Immunization status and hospitalization for vaccine-preventable and non-vaccine-preventable infections in liver-transplanted children [J] . Palittiya Sintusek ,Yong Poovorawan . 世界肝病学杂志：英文版（电子版） . 2021,第1期
• 2. Can the detection of IgA anti-Mycoplasma pneumoniae added to IgM increase diagnostic accuracy in patients with infections of the lower respiratory airways? [J] . Massimo De Paschale ,Teresa Cerulli ,Debora Cagnin . 世界临床传染病学杂志 . 2016,第4期
• 3. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 4. 鸡球虫病疫苗不同免疫接种方法的分析比较 [C] . . 首届中国兽药大会暨中国畜牧兽医学会动物药品学分会2008年学术年会 . 2008
• 5. 鸭疫里默氏杆菌菌蜕疫苗制备方法的研究及免疫效果的评价 [A] . 董洪亮 . 2013