A procedure for the preparation of a microbial insecticide. (Machine-translation by Google Translate, not legally binding)

摘要

A method for preparing a microbial insecticide from an insecticide-producing spore-forming microorganism of the type that sporulates and also forms crystals, preferably bacillus thuringiensis bacillus or bacillus sotto, characterized by effecting sporulation and crystal formation under conditions submerged aerobes, preferably for a period of 14 to 32 hours in an aqueous fermentation medium containing balanced amounts of fermentable carbohydrates and assimilable nitrogen so that they are exhausted within 6 hours of difference between one and the other after the start of sporulation, for example by the use of 0.6 to 2% beet molasses, from 1 to 1.5% of an oil-free cottonseed endosperm or cottonseed meal, and from 0.8 to 1, 7% of corn infusion solids, preferably in the presence of a regulator to maintain the ph of the medium between 7.2 and 7.6 and isolate the spores and the crystals thus produced. (Machine-translation by Google Translate, not legally binding)