Organic cpds. prepn. by co-enzymatic reduction - with continuous regeneration of the oxidised coenzyme with a reducing agent

摘要

The matls. reduced contain aldehyde, ketone, acid, imine, quinone, quinone-imine, hydroxy acid, hydroxy aldehyde gps., and are reduced by an enzyme and a coreactor (I) (coenzyme) which is regenerated by redn. with cpd. (II) contg. alcohol, aldehyde, acid, imine, thiol functions. (I) is nicotinamide adenin dinucleotide (phosphate) or flavin adenin dinucleotide. (II) is an alcohol (ethanol) in presence of ethanol dehydrogenase. pH of reaction mixt. is 8.0-9.0, reaction temp. is room temp. -40 degrees C. The process is used for reductive amination of alpha-ketoglutaric acid or pyruvic acid, or reductive carboxylation of amino acetone.