A process for the in vitro - propagation and in vitro - maintaining of cells

摘要

The propagation and/or maintenance of living cells in vitro is accomplished by attaching and growing cells on one side or surface of a hollow fiber membrane. The attached cells are propagated and/or maintained by passing oxygen through the membrane from the other side and into contact with the cells and simultaneously incubating the cells in a nutrient medium. This method is employed in tissue and cell culturing of altered and unaltered cells which attach to a surface for maintenance and growth.