still for framstellning of monensinmetabolit a 27106 or acid, ammonium, lithium, potassium, rubidium or cesiumformerna derav, whereby monensin and glucose is converted to hjelp of streptomyces candidus.

摘要

1457796 Metabolite A-27106 ELI LILLY & CO 30 Jan 1974 [1 Feb 1973] 04414/74 Headings C2A and C2C The invention relates to Metabolite A-27106 and the acid, ammonium, lithium, potassium, rubidium or cesium forms thereof. The metabolite is a white crystalline solid with (a) a molecular weight of 854, as determined by mass spectrometry; (b) an elemental composition of 58À78% carbon, 8À51% hydrogen, 27À85% oxygen and 3À63% sodium; (c) an empirical formula of C 42 H 71 O 16 Na; (d) an infra-red absorption spectrum in chloroform as shown in the accompanying drawing; (e) a mass spectrum having a molecular-ion peak at m./e. 854À44630 and characteristic peaks at m./e. 836À44129, 779À44690, 761À44740; and (f) an Rf value of 0À49 on silica gel thin layer chromatography in benzene-methanol (7 : 3); and the acid form is a white solid having a molecular weight of 832 and a titratable group with a pKa value of 7À2. The following structure is proposed for Metabolite A-27106. Metabolite A-27106 is prepared by cultivating Streptomyces candidus NRRL 5449 in a culture medium containing assimilable sources of carbon, nitrogen and inorganic salts under submerged aerobic conditions in the presence of glucose and monensin until monensin is converted into metabolite A-27106. The metabolite may be mixed with a physiologically-acceptable carrier, e.g. poultry feed, and administered to poultry for preventing or treating coccidiosis therein. The metabolite may also be mixed with ruminant feed for increasing the efficiency of feed utilization of ruminant animals.