Method of identification of a protein of the surface of the cells cancerous, clinical trial and advantages of immunization

摘要

P the present invention relates to the detection and the prevention of certain cancers. / p & & p & it is a method of identification of the antigenic protein of neoplastic cell surface, causing a specific cellular immunity, while maintaining the biological activity of the protein, comprising the following steps: a) the separation of neoplastic cells for analysis; b) the separation and suspension of the glycoproteins in an aqueous solution; c) separation of the glycoproteins containing more than 60% of carbohydrates, the solution by extraction with phenol very hot, involving the processing of the solution with phenol heated, to cool and to remove the phenol of the sample, and then to be treated with alcohol, to cool and to remove the precipitate, which contains the antigenic protein of the cell surface neoplasm, d) separation of the antigenic protein of the cell surface neoplastic by the process of the antigenic concanavaline a surface on a surface containing the concanavaline a, to connect the protein by concanavaline a and then move the antigenic protein of the cell surface neoplastic of the sugar solution. The present invention makes it possible, in particular, the detection of the ductal carcinoma. / p