Separation of the isomers of tocopherol by liquid/solid chromatography

摘要

Methods of separating the alpha-, beta-, gamma-, and delta- tocopherol isomers from mixtures containing these isomers by liquid/solid chromatography techniques using a liquid phase comprising chloroform substantially free of ethanol are disclosed. These methods are particularly suitable for the quantitative determination of these isomers in vegetable oils and in margarines, and for the isolation of substantially pure isomeric forms from naturally occurring mixtures and from the by-products of crude edible oil refinement.