An antigen preparation for use in the detection of syphilis and other treponemal diseases comprising (1) V.D.R.L. or U.S.R., antigen reagent and (2) A. A COMPOUND HAVING THE FORMULA B. A COMPOUND HAVING THE FORMULA C. A COMPOUND HAVING THE FORMULA OR (D) MIXTURES OF (A) + (B) OR (A) + (C), AND (3) WHICH MAY ADDITIONALLY INCLUDE A POLYMERIC FLOCCULATING AGENT. A method of using said antigen preparation and a test card having a flocculating agent deposited thereon, are also disclosed.
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