PURPOSE:To detect immunologically trace components with high reproducibility and sensitivity by the method wherein an antigen or antibody labelled with a silver halide fogging agent is subjected to the immunoreaction, the labelled substance which has not undergone the reaction is separated from one which has undergone the reaction, and silver halide is developed in the presence of either one of them. CONSTITUTION:In detecting immunologically trace components of insulin, growth hormone, or the like, an antigen or antibody is labelled with a fogging agent capable of fogging silver halide, such as dithiocarbomate, hydrazine, or thiourea, and is subjected to immunoreaction. Next, the labelled antigen or antibody which has not undergone the reaction is separated from the labelled antigen-antibody reaction product, and silver halide is developed in the presence of either one of them. Then the photographic density is measured and is compared with the calibration curve prepared in advance, to determine the amounts of the trace components.
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