METHOD FOR DIAGNOSTIC TESTS IN VITRO IN ORDER TO DISCOVER MALIGNANT NEOPLASMS PARTICULARLY AT HUMAN PATIENTS AND A TESTING PLATE FOR SAME

摘要

An in vitro testing method for the diagnosis of malignant tumours preferably human tumours, by determining the mobility variation of cellular blood components contacted with a migration inhibition factor (MIF), the MIF being secreted from lymphocytes being sensitized against tumour tissue, comprises subjecting, as said cellular blood components, the lymphocytes contained in a leucocyte suspension to be examined to a migration inhibition test. The lymphocytes are contacted with an antigen capable of stimulating them to produce MIF. The leucocyte migration is inhibited and fixed after a predetermined period of time. The leucocytes migrate in a capillary intermediate layer having a thickness of from 1 to 20 microns and being at least partially filled with liquid. Said intermediate layer is formed between a glass surface and an agar layer. The invention further refers to the construction and preparation of a testing plate or dish comprising these layers.