process for extracting solvents and in particular improvements in the praparazione of protein concentrate of fish with this method

• 1. 对整联蛋白,特别是ανβ3整联蛋白具有特异性活性的三环化合物,它们的制备方法和这种方法的中间体，它们作为药物的用途以及含有它们的药物组合物 [P] . 中国专利： CN1090178C . 2002.09.04
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  机译： 贝类提取物浓缩物的制备方法以及贝类提取物浓缩物

• 4. Process for preparation of shellfish extract concentrate, and shellfish extract concentrate [P] . 美国专利： US4968523A . 1990-11-06

  机译： 贝类提取物浓缩物的制备方法以及贝类提取物浓缩物

• 5. Process for preparation of shellfish extract concentrate, and shellfish extract concentrate [P] . 欧洲知识产权局专利： EP0300747A1 . 1989-01-25

  机译： 贝类提取物浓缩物的制备方法以及贝类提取物浓缩物

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• 5. OPTIMUM PROTEIN REQUIREMENTS AND OPTIMUM RATIO BETWEEN ANIMAL PROTEIN TO PLANT PROTEIN IN FORMULATED DIETS FOR NILE TILAPIA (TILAPIA NILOTICA L.) (SOYBEAN CONCENTRATE, ANCHOVY FISH MEAL, DIGESTABILITY, SEMI-PURIFIED DIETS, FISH NUTRITION). [D] . ABDELGHANY, ALI EZZELDIN. 1986
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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Preparation of Soybean Protein Concentrate with Mixed Solvents of Hexane－Aqueous Alcohol [J] . ZhangWeinong ,LiuDachuan . 中国粮油学报 . 2002,第G00期
• 3. 临床化学方法的分类、特别是自动生化仪方法的特点 [C] . 杨振华 . 中国化工学会试剂学会第11次学术交流会 . 1988
• 4. 物理方法改进水溶性猪肝蛋白的乳化特性及在肉制品中的应用研究 [A] . 丁景 . 2019