Novel antibiotic, OS-3966-A (I) is produced by aerobic culture of Streptomyces rosa var. notoensis (FERM-P2209). In an example, microorganism was cultured on a medium (pH 7.0) contg. glycerol 2.0, soybean powder 2.0, and NaCl 0.3% at 27 degrees C for 4 days. (I) in the culture filtrate was extracted with BuOAc at pH 2.0, transferred to 1% NaHCO3, extracted with EtOAc at pH 2.0, concd., and ppted. by addn. of petroleum ether. The yellowish brown powder was purified by a silica gel chromatography yielding 317 mg. crystals from 20 l. broth. (I) had molecular formula C16H14O6, m.pt. 178-180 degrees C, and alpha 26d = -27.5 degrees. (I) was soluble in MeOH, EtOH, EtOAc, CHCl3, Me2CO, and Et2O and insoluble in hexane, petroleum ether, and water. (I) was +ve to FeCl3 and reductive catalysts reactions and -ve to ninhydrin, Sakaguchi's, Ehrlich's, Fehling's, and Molish's reactions. (I) was effective against Mycoplasma, Gram-positive bacteria, and Trichophyton. (I) had an LD50 for mice of 282 mg./-kg. mice by i.p. injection.
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