Quantitative gas chromatographic determn. of enantiomers - by mixing sample with reference enantiomer before analysis and comparing the ratio of the areas under the peaks

摘要

Appts. for the quantitative gas chromatographic determn. of enantiomeric substances such as amino acids, amines, aminoalcohols, hydroxycarboxylic acids and glycols comprises a dosing system, oven, detector and recording system equipped with a gas chromatography column charged with the chiral polysiloxane described in the parent patent as the stationary phase. Process for quantitative gas chromatographic determn. of enantiomeric cpds. comprises mixing the sample with a reference enantiomer before analysis, separating the enantiomers in the appts. and then quantitatively determining the enantiomers by comparing the ratio of the areas under the peaks. Routine, automated quantitative analysis of amino acids in the region of 100 pico-mol. can be carried out with great accuracy.