Vectors, the lambda bacteriophage genomes, allowing the insertion and the expression of a gene or prokaryotic or eukaryotic, under the control of the promoter and of the operator of the lactose operon bacterium escherichia coli

摘要

P dna of bacteriophage lambda . It comprises mutations qam73 and sam7 having the effect of preventing or delaying the expression of the late genes, a dna fragment comprising at least a portion of the gene in escherichia coli and a promoter of a bacterial operon, in particular of the lactose operon in a non-essential portion of the genome of the phase, in the gene z, a cleavage site by endonuclease such as ecori, to the exclusion of any other site of the same type in the dna of phage. / p & & p & application to the induction by a bacterium of protein synthesis, which is directed. / p