A culture collection and transport device which is uniquely configured employing a piston-like element which is adapted to maintain a medium-carrying chamber in fluid-tight relationship until such time as a specimen sample is introduced into the device and selectively put in contact with the medium. The culture medium preserves the viability of microorganisms comprising the specimen sample. The piston-like member is pressure responsive so that ambient temperature and pressure changes will not cause leakage of the medium from the medium-carrying chamber and wherein a simple, force oriented movement of a specimen collector on swab, containing a culture, is readily put into contact with the viability-maintaining, transport medium.
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