A method for the production of biocatalysts with high mechanical strength and high load of enzymatically active substance and of bead biocatalyst

摘要

1. A method for producing biocatalysts having high mechanical strength and heavily loaded with enzymatically active substance by polymeric inclusion of biomass, by using the mixture (A) of a suspension or solution of an enzymatically active substance in an aqueous solution of a polyelectrolyte, characterized in that the concentration of the polyelectrolyte lies in a range between 0.5 to 15 % by weight, and the dissolution of a salt having polyvalent ions charged oppositely to the polyelectrolytes in a concentration range of 0.02 to 35 % by weight is used as a precipitation bath (B), and in stage 1, the mixture (A) being injected into the mixture (B) by forming beadlike particles in a grain-size range of about 0.5 to about 10 mm, these being solidified by slow, uniform stirring for about 0.5 to 4 h, and in stage 2, the catalyst beads thus formed being filtered out with the enclosed enzymatically active substance, being washed with a physiological salt solution or the aqueous solution or emulsion of a protective colloid, and in stage 3, by passing thereover air at a temperature of up to 80 degrees for up to about 48 h, being dried by shrinking, and in stage 4, the catalyst beads being introduced into an aqueous solution or emulsion of a protective colloid, thus hardening the enzyme system and the network, and thereafter being discharged after about 24 h in the moist condition.