QUANTITATIVE DETERMINATION OF TSH IN AQUEOUS SOLUTIONS BY RADIOIMMUNOASSAY

摘要

An improved immunoassay method for determining thyroid stimulating hormone (TSH) in a liquid sample wherein the bound- and free- species of the labelled, preferably radio-labelled, TSH are separated by contacting the liquid reaction mixture with Florisil adsorbent which selectively adsorbs free TSH and physically separating the adsorbent from the liquid mixture. The Florisil adsorbent is preferably contained in a column form. A test kit for performing the improved method is also provided. The assay is more simple and less time consuming than the prior art techniques.