LECTIN IMMUNOASSAY FOR THE DETERMINATION OF TERMINAL SACCHARIDE GROUPS IN GLYCOPROTEIN

摘要

A method of determining qualitatively and/or quantitatively terminal saccharide groups in glycoproteins from body liquids having a microheterogeneity in the saccharide group or groups. The method comprises three steps: 1) Binding of glycoprotein antibodies to a solid phase; 2) Binding of glycoprotein from body liquid and from standard solutions with a defined composition of terminal saccharride groups to the product from step 1; 3) Binding of labeled lectin to the complex of antiglycoprotein, glycoprotein and solid phase from step 2. The amount of bound labeled lectin can then be measured in the product from step 3 or the amount of unbound marked lectin in the supernatant and possible washing solutions can be measured. This amount is correlated to the amount of terminal saccharide groups in the glycoprotein and on the basis of this the amount of a glycoprotein component with a special composition of terminal saccharide groups can also be determined. The method can be used in medical diagnostics.