C1-Esterase inhibitor activity determn. e.g. in plasma - by spectrophotometric analysis of chromogenic peptide substrate cleavage prods. (AT 15.04.82)

摘要

In a new procedure for the determn. of the C1-esterase inhibitor (CEI) activity of CEI-contg. specimens by comparison of the C1-esterase activity of a standardised C1-esterase prepn. with that of the same standardised prepn. in the presence of the CEI-contg. specimen, the C1-esterase activity is determined by spectrophotometric examination of cleavage prods. of chromogenic peptide derivs. R1-A-B-NH-R (I) (where R1 is H, alkyl, a sulphonyl residue (esp. benzenesulphonyl or p-methoxy-benzenesulphonyl) or acyl (esp. alkoxycarbonyl or formyl); A is an amino acid component or di- or tripeptide residue; B is D-Arg, L-Arg, D-Lys, L-Lys, D-Orn, L-Orn, D-Tyr, L-Tyr, D-Ala or L-Ala; and NH-R is a chromophoric or fluorescent gp.). Used for determin. of C1-esterase inhibitor activity in human and animal body fluids.