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Process for the manufacture of hybrid cells producing an antibody in which methylcellulose is used in the selection of hydrides
Process for the manufacture of hybrid cells producing an antibody in which methylcellulose is used in the selection of hydrides
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机译:用于生产杂交细胞的方法,该杂交细胞产生一种抗体,其中甲基纤维素用于选择氢化物
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摘要
A process for the manufacture of hybrid cells capable of producing an antibody in which (a) mice are immunised with an antigen, (b) a lymphocyte mixture is isolated from the mice, (c) B lymphocytes are fused or hybridised with mouse B myeloma cells, (d) the mixture consisting of formed hybrids and non-hybridised B myeloma cells is conveyed into a culture medium for the formed hybrids and the formed hybrids are cultivated and selected with respect to the non- hybridised B myeloma cells, (e) they are subcloned to provide hybrids which are capable of producing the antibody which is directed specifically against the antigen, and (f) optionally the hybrids are isolated, characterised in that at step (d) the selection process is carried out in a medium containing methylcellulose (as hereinbefore defined), and hybrids selected at step (d) are optionally injected into the abdominal cavity of mice (which have previously been treated with a tumour stimulant), multiplied hybrids are removed from the abdominal cavity and subsequently the subcloning and isolating steps (d) and (f) are carried out. The invention also provides a process for producing monoclonal antibodies, and further provides hybrid cells capable of producing antibodies directed specifically against human interferon and provides such monoclonal antibodies.
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