Immunoradiometric assay employing terminal radionuclide labeling and synthesis of conjugates for such assay

摘要

An immunoradiometric assay in which an antigen is reacted to its specific antibody, which complex is then separated and further reacted with a second, universal antibody. This second, universal antibody contains a chelating moiety, such as transferrin, capable of binding specifically and strongly short-lived radionuclides. The second complex, once formed is reacted with a radionuclide and the radio- activity is measured, determining thereby the quantity of the unknown antigen originally present.