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Method of and reagents for quantitative analysis of cyclic nucleotides
Method of and reagents for quantitative analysis of cyclic nucleotides
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机译:定量分析环核苷酸的方法和试剂
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摘要
A method of quantitative analysis of cyclic nucleotides which comprises: acylating a cyclic nucleotide in an assay sample by using an acylating agent; causing said acyl-cyclic nucleotide and a specific quantity of an enzyme-labeled cyclic nucleotide formed by bonding a cyclic nucleotide corresponding to the acyl-cyclic nucleotide and an enzyme through a dicarboxylic acid to undergo competitive reaction with a specific quantity of an antibody with respect to a corresponding cyclic nucleotide; separating the enzyme-labeled cyclic nucleotide which has become bonded to said antibody and the enzyme-labeled cyclic nucleotide which has not become bonded thereto; and determining the quantity of the cyclic nucleotide by measuring the enzyme activity of either of the enzyme-labeled cyclic nucleotides. For this analysis, a reagent comprising an enzyme-labeled cyclic nucleotide is used.
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