ESTABLISHMENT OF CELL STRAIN PRODUCING HUMAN PITUITARY HORMONE

摘要

PURPOSE:To establish the titled cell strain stable for a long period, by culturing the tissue of human pituitary gland in a specific medium, transplanting the resultant epithelial cells in the body of an animal having lowered immunity, extirpating the proliferated cells, and culturing the extirpated cells. CONSTITUTION:The tissue of human pituitary cells extirpated from human body (e.g. extirpated from the patient of gigantism or acromegaly, etc.) is dispersed in a solution of proteolytic enzyme (e.g. dispase, etc.: the concentration of the solution is 50-1,200 protease unit/ml). The dispersed cells are added to a cultivation liquid containing blood serum and Ham F-10 medium and cultured in the medium. The proliferated epithelial cells are transplanted in the body of an animal having lowered immunity and proliferated in the body. The cells are extirpated from the body, and dispersed and cultured again in the solution of proteolytic enzyme and the above cultivation liquid to obtain established cells originated from human pituitary gland tissue.