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Process for the purification of urokinase present in a crude urokinase-containing solution with the aid of a strongly acidic cation exchanger having sulphonic acid groups
Process for the purification of urokinase present in a crude urokinase-containing solution with the aid of a strongly acidic cation exchanger having sulphonic acid groups
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机译:借助具有磺酸基团的强酸性阳离子交换剂,纯化含尿激酶的粗溶液中存在的尿激酶的方法
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摘要
A process for the purification of urokinase by treating a solution containing crude urokinase with a strongly acidic cation-exchange resin that contains sulphonic acid groups and has been converted into the NH4(+) form. The urokinase is adsorbed on this cation-exchange resin and is then eluted from it. Before this step, however, urea is added to the crude urokinase-containing solution in such an amount that the final urea concentration of the solution is 3-8 M. This gives a purified urokinase with an essentially constant and high specific activity, using a crude urokinase-containing starting solution, and the yield is high, irrespective of the purity of the crude urokinase solution employed.
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