METHOD OF OBTAINING STREPTOMYCES PROTOPLASTS CAPABLE OF EFFICIENT CELL REGENERATION

摘要

The efficiency of cell regeneration from protoplasts of Streptomyces fradiae and S. griseofuscus is growth-phase dependent; max. regeneration is obtained from cells harvested between late exponential and stationary growth phase. Recombination frequencies in intraspecific streptomycete croses by protoplast fusion are very high and typically vary from -0.5 to 15% of the total viable protoplasts. The frequency of recombinants can be further increased in S. coelicolor and S. fradiae(and presumably in other Streptomyces) by treating the protoplast with UV light.