YEAST DEVELOPABLE VECTOR PLASMID UTILIZING PROMOTOR FOR GENE CONTROLLING GLYCOLYTIC ENZYME AND METHOD FOR UTILIZING THE SAME

摘要

PURPOSE:To produce the aimed useful peptide in high yield, by utilizing a promoter of an operon controlling 3-phosphoglycerokinase. CONSTITUTION:A plasmid containing a promoter for a gene controlling 3-phosphoglycerokinase (PGK) and the aimed peptide is prepared, and a yeast transformed by the above-mentioned plasmid is cultivated. alpha-Neoendorphin (alphaNE) gene is used as the aimed peptide gene, and the aimed substance (alphaNE) is produced as a PGK-alphaNE hybrid protein from the sequence such as PGK promoter- PGK structure gene-alphaNE gene. A gene capable of coding the aimed gene, e.g. a peptide such as interferon, is added to a site at a distance of a suitable base sequence from the position of the PGK promoter, and the aimed peptide can be directly produced.