Method clarifies huminous water and high concentration huminousus substances

• 1. 一种水相电解质中对固态腐殖质进行循环伏安分析的方法 [P] . 中国专利： CN108519424B . 2019.06.28
• 2. 一种水相电解质中对固态腐殖质进行循环伏安分析的方法 [P] . 中国专利： CN108519424A . 2018-09-11
• 3. METHOD OF CLARIFICATION OF HUMINOUS WATER WITH HIGH CONCENTRATION OF HUMINOUSUS MATTER [P] . CS231472B1 . 1984-11-19

  机译： 高浓度腐殖质澄清水的方法

• 4. Method of preparing a composition enriched in phenolic compounds with a molecular Mass less than 400, from Olive vegetation Water, including clarification of these waters, the waters of vegetation tangential ultrafiltration Nanofiltration clarified, permeate, adsorption of phenolic compounds You and Concentration. [P] . CL2011002021A1 . 2012-01-27

  机译： 从橄榄植物水中制备富含分子量小于400的酚类化合物的组合物的方法，包括对这些水进行澄清，对植物进行切向超滤的水进行纳滤，澄清，渗透，酚类化合物You的吸附和浓缩。

• 5. Water-soluble organic substance concentration method and water-soluble organic substance concentration apparatus [P] . 日本专利： JP6196807B2 . 2017-09-13

  机译： 水溶性有机物浓缩方法及水溶性有机物浓缩装置

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• 4. The Development of a Fluorescence-based Reverse Flow Injection Analysis (rFIA) Method for Quantifying Ammonium at Nanomolar Concentrations in Oligotrophic Seawater [D] . Abbott, William R. T. 2015
• 5. Effectiveness of the mixture of nopal and cassava starch as clarifying substances in water purification: A case study in Colombia [O] . José Lugo-Arias, Elkyn Lugo-Arias, David Ovallos-Gazabon, 2020
• 6. A Method for Quantitative Analysis of Aquatic Humic Substances in Clear Water Based on Carbon Concentration [O] . Kumiko TSUDA, Akihiro TAKATA, Hidekado SHIRAI, 2012
• 7. Evaluation of Methods for the Isolation or Concentration of Organic Substances from Water Using XAD-4 Quaternary Resin [R] . Ben-Poorat, S. , Kennedy, D. C. , Byington, C. H. 1985

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Evaluation of Size Structure in Freshwater Cyanobacterial Populations: Methods to Quantify Risk Associated with Changes in Biomass and Microcystin Concentrations [J] . Nancy J. Leland ,James F. Haney ,Kristin Conte . 水资源与保护（英文） . 2019,第6期
• 3. Electromagnetic and Optical Methods for Measurements of Salt Concentration of Water [J] . Ф.A.Tengesdal ,B.L.Hauge ,L.E.Helseth . 电磁分析与应用期刊（英文） . 2014,第6期
• 4. 腐殖质原矿和商品中胡敏酸和富里酸定量分析的一种新标准化方法 [C] . Richard T.Lamar ,Richard T.Lamar ,Daniel C.Olk . 首届全国土肥和谐大会暨第二届中国泥炭产业高峰论坛 . 2016
• 5. 催化臭氧去除垃圾渗滤液中DEHP及高浓度腐殖质的机理研究 [A] . 刘卫华 . 2007