Process for the preparation of human tissue plasminogen activator (TPA) in host cells, expression vector for t-PA and DHFR protein, cells transformed with the vector, and method for enhancing the production of t-PA vaertscellernes

摘要

A method and vectors for producing tissue plasminogen activator (tPA) in eukaryotic host cells is disclosed. Enhanced levels of tPA production are obtained by co-amplification of the tPA gene through treatment with methotrexate of cultures transformed with expression vectors coding additionally for mutant or wild type DHFR.