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AN IMPROVED METHOD OF DETECTION IN A CHROMATOGRAPHIC ANALYSIS OF A PHYSIOLOGICAL SAMPLE FOR A PHARMACY.
AN IMPROVED METHOD OF DETECTION IN A CHROMATOGRAPHIC ANALYSIS OF A PHYSIOLOGICAL SAMPLE FOR A PHARMACY.
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机译:一种用于制药的生理样品色谱分析中的改进检测方法。
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摘要
A chromatographic plate for use in thin-layer chromatography for the detection of drugs in a physiological medium comprises an inert substrate and an adsorbent material impregnated with a heavy metal oxidising agent, preferably a vanadium salt, which chemically reacts with a broad range of drugs, preferably basic amine-containing drugs, in the presence of a strong acid reagent, preferably concentrated sulphuric acid, to produce a characteristic colour on the chromatographic plate. The steps of extracting the drug from the physiological medium, introducing the drug to the chromatographic plate and developing the chromatographic plate are conventional, but by including the heavy metal oxidising reagent on the chromatographic plate the detection of the drugs can be carried out simply by exposure to the strong acid reagent.
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