METHOD FOR DIAGNOSIS OF MASTITIS USING ENZYME, N-ACETYL-B-D -GLUCOSAMINIDASE

摘要

A method for diagnosis of mastitis in cattle by a colorimetric determination of the enzyme N-ACETYL- beta -D-GLUCOSAMINIDASE (NAGase) in milk samples. The method comprises - (1) adding a milk sample to a preheated aqueous substrate/buffer solution comprising a substrate having the formula CHEM and an acid/anion buffer, (2) incubating the mixture for a suitable period then stopping the reaction, (3) conducting a colorimetric comparison between sample and standard. The invention also includes a test kit for carrying out the method.