Observation of immunofluorescene for distinguishing between specific and nonspecific binding of conjugates

摘要

Disclosed are a method and a device for distinguishing particles or regions therein to which fluorochrome-labeled antibodies or antigens, termed conjugates, are bound by the specific immune reaction of an antibody with its homologous antigen from particles or regions to which the conjugates are nonspecifically bound. The method is based on the different bleaching behavior caused by the different types of binding. Use is made of a microscope whose entire field of view is conventionally illuminated, a central laser beam serving for bleaching analysis.