IMMOBILIZATION OF MICROBIAL CELL WITH ACRYLAMIDE

摘要

PURPOSE:To produce spherical immobilized microbial cell having high strength, and capable of keeping high activity for a long period, by including a microbial suspension containing acrylamide and a crosslinking agent in a coating film of an alginic acid salt, and polymerizing the acrylamide. CONSTITUTION:A microbial suspension liquid contaning 10-35% acrylamide, 0.5-6% crosslinking agent and 0.5-2.1% alginic acid salt such as sodium alginate, potassium alginate, etc. is added dropwise to a solution of a calcium salt and/or an aluminum salt such as calcium chloride, aluminum sulfate, etc. The mixture is thrown into an organic solvent such as benzene, ether, etc., and acrylamide is polymerized in the solvent.