ANTI-HUMAN LACTOFERRIN ANTIBODY SENSITIZED BLOOD CELL AND DETECTION OF HUMAN LACTOFERRIN USING SAID BLOOD CELL

摘要

PURPOSE:To obtain an index useful for diagnosis of a pancrea disease by conjugating an anti-human lactoferrin antibody with fixed blood via tannic acid to manufacture anti-human lactoferrin antibody sensitized blood cells and bringing such sensitized blood cells and the pancreatic juice into contact with each other. CONSTITUTION:The anit-human lactoferrin (Lf) antibody is obtd. with an animal such as guinea pig processing antibody production ability by using human colostrum and is refined. Tannic acid is then added to the fixed blood of a goat, etc. to conjugate the binder to the blood cell surface and thereafter a liquid contg. the anti-human Lf antibody is brought into contact therewith, by which the anti- human Lf antibody sensitized blood cells are obtd. A specified quantity of the diluted pancreatic juice and standard human Lf are dispensed on a microplate to make a dilution system and a specified quantity of the anti-human Lf antibody sensitized blood cells are added thereto to make the system uniform. The concn. of the human Lf is measured by hemagglutination after resting for specified time. The examination of the pancrea disease is thus elucidated.