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Immunoassay method for a iodothyronine in a biological fluid and the reagent system, test kit and test device for use in the method
Immunoassay method for a iodothyronine in a biological fluid and the reagent system, test kit and test device for use in the method
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机译:生物体液中碘甲状腺素的免疫测定方法以及该方法中使用的试剂系统,检测试剂盒和检测装置
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摘要
An improved immunoassay method, reagent means, test kit, and test device for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid (HMS), or a salt thereof, is employed as a blocking agent for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays employing labels which are participants in enzyme-catalyzed reactions. Such labels include enzyme substrates, coenzymes, enzyme inhibitors, enzyme prosthetic groups, and enzymes.
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