method for the selective extraction of vinblastine and vincristine, vinleurosine respectively. sulfates by extraction of parts of vinca rosea l.

摘要

Vinblastine (I), vinleurosine (II) and vincristine (III) or their salts, known cytostatic agents, are recovered in improved yields from Vinca rosea L by (a) extracting with neutral aqs. methanol, (b) dissolving the alkaloid mixture obtained in a known manner from the extract in ethanol contg. is not 10% acid and crystallising (I)-(III) from the ethanol soln. in the form of their acid addition salts; (c) chromatographing the mixture of acid addition salts, opt. after conversion into the free bases, on Al2O3 partially deactivated with water and fractionally eluting, and (d) recovering (I)-(III) or their acid addition salts from the eluate fractions and purifying them if required. The process involves less phase interchanges than prior methods and the chromatographic separation is less complicated.